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Related Experiment Videos

A new storage medium for canine blood.

J E Smith, E Mahaffey, P Board

    Journal of the American Veterinary Medical Association
    |March 15, 1978
    PubMed
    Summary

    A new canine blood storage solution extends shelf life up to six weeks. This preservation method ensures sufficient 2,3-diphosphoglycerate levels for safe canine blood transfusions.

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    Area of Science:

    • Veterinary Medicine
    • Biochemistry
    • Hematology

    Background:

    • Extending the shelf life of stored canine blood is crucial for transfusion efficacy.
    • Current storage solutions may limit the viability and oxygen-carrying capacity of banked blood.

    Purpose of the Study:

    • To develop and evaluate a novel storage solution to prolong the viability of canine erythrocytes.
    • To assess the impact of the storage solution on 2,3-diphosphoglycerate (2,3-DPG) levels and oxygen dissociation.

    Main Methods:

    • A storage solution containing ascorbate phosphate, citric acid, sodium citrate, sodium phosphate, and dextrose was formulated.
    • Canine blood was stored at 4°C in the developed solution.
    • Post-storage viability and 2,3-DPG concentrations were measured over a six-week period.

    Main Results:

    • Canine blood stored in the solution maintained over 70% viability at 24 hours post-storage for up to six weeks.
    • 2,3-DPG levels remained stable for three weeks and then declined slowly.
    • After six weeks, 2,3-DPG levels were sufficient for adequate in vivo oxygen release from oxyhemoglobin.

    Conclusions:

    • The developed storage solution effectively extends the shelf life of canine blood up to six weeks.
    • Blood stored for six weeks in this solution is considered safe for transfusion due to maintained 2,3-DPG levels.
    • This advancement offers a promising option for canine blood banking and transfusion practices.

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