Related Experiment Video
Updated: Aug 14, 2026
08:02
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Published on: January 22, 2020
[Release in secondary mixed leukocyte cultures of a factor inducing citrulline production by bone marrow cells]
Comptes Rendus Des Seances De L'Academie Des Sciences. Serie III, Sciences De La Vie
|January 1, 1983
Abstract:
Citrulline production is demonstrated in murine bone marrow cell cultures incubated with dialyzed secondary MLC supernatant. This synthesis, proceeding from arginine, is induced by a soluble protein factor released during secondary MLC, unstable to heat, with a molecular weight of approximately 150,000 daltons.
Insights
Murine bone marrow cells synthesize citrulline from arginine. This process is triggered by a heat-unstable protein factor found in secondary mixed lymphocyte culture (MLC) supernatant.
Area of Science:
- Biochemistry
- Immunology
- Cell Biology
Context:
- Investigates citrulline synthesis in murine bone marrow cells.
- Utilizes dialyzed secondary mixed lymphocyte culture (MLC) supernatant.
- Examines the role of soluble protein factors in cellular metabolism.
Purpose:
- To demonstrate and characterize citrulline production in bone marrow cells.
- To identify the inducer of citrulline synthesis.
- To determine the properties of the inducing factor.
Summary:
- Citrulline production was observed in murine bone marrow cell cultures.
- The synthesis originates from arginine.
- A heat-unstable soluble protein factor (approx. 150,000 daltons) from secondary MLC supernatant induces this synthesis.
Impact:
- Elucidates a novel pathway for citrulline production in hematopoietic cells.
- Identifies a specific protein factor regulating this metabolic pathway.
- Provides insights into cell-cell communication and metabolic regulation in immune responses.
