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Related Experiment Video

Updated: Jun 17, 2026

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
13:20

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Published on: July 30, 2010

Immunoglobulin gene transcription is activated by downstream sequence elements.

C Queen, D Baltimore

    Cell
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Researchers identified DNA sequences crucial for kappa immunoglobulin gene expression regulation. These sequences, located downstream of the J kappa region, are essential for accurate gene transcription in myeloma cells.

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    Published on: March 7, 2018

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Kappa immunoglobulin gene expression is tightly regulated.
    • Understanding regulatory DNA sequences is key to controlling antibody production.

    Purpose of the Study:

    • To identify and characterize DNA sequences regulating kappa immunoglobulin gene expression.
    • To determine the role of downstream sequences in kappa gene transcription.

    Main Methods:

    • Development of a transient expression assay for detecting kappa gene transcription.
    • Transfection of cloned, rearranged kappa genes into mouse myeloma and monkey cell lines.
    • Deletion analysis of the kappa gene to identify regulatory elements.

    Main Results:

    • Transcription was detected from a rearranged kappa gene in myeloma cells.
    • Deletion of downstream sequences abolished transcription in myeloma cells, indicating their necessity.
    • Transcription was also detected in monkey cells but was lower and independent of downstream sequences.

    Conclusions:

    • A DNA sequence downstream of the kappa variable region promoter is essential for accurate transcription initiation in myeloma cells.
    • These downstream sequences play a critical role in regulating kappa immunoglobulin gene expression.