Related Experiment Video

Updated: May 11, 2026

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro
09:16

Analysis of RNA Processing Reactions Using Cell Free Systems: 3' End Cleavage of Pre-mRNA Substrates in vitro

Published on: May 3, 2014

Bacillus subtilis RNAase III cleavage sites in phage SP82 early mRNA

A T Panganiban, H R Whiteley

    Cell
    |July 1, 1983
    PubMed

    Insights

    Researchers identified specific DNA sequences in Bacillus subtilis phage SP82 mRNA targeted by a processing endonuclease. These sites, forming stem-loop structures, are cleaved at adenosine residues, offering new insights into RNA processing.

    Area of Science:

    • Molecular Biology
    • Virology
    • Biochemistry

    Background:

    • Bacterial endonucleases play crucial roles in RNA processing.
    • Understanding specific cleavage sites is key to deciphering gene regulation.

    Purpose of the Study:

    • To determine the precise DNA sequences and RNA structures involved in Bacillus subtilis phage SP82 mRNA processing.
    • To characterize the cleavage mechanism of a B. subtilis processing endonuclease.

    Main Methods:

    • DNA sequencing of cleavage products.
    • RNA secondary structure analysis (stem-loop formation).
    • S1 mapping experiments using RNA-DNA hybrids.

    Main Results:

    • Identified three specific cleavage sites in SP82 mRNA.
    • Proposed a model where cleavage occurs at adenosine residues within stem-loop structures.
    • Confirmed identical cleavage sites in vivo and in vitro.

    Conclusions:

    • The B. subtilis endonuclease recognizes double-stranded RNA structures.
    • SP82 mRNA processing sites exhibit unique features compared to E. coli RNAase III sites.

    More Related Videos

    Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo10:51

    Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

    Published on: October 31, 2014

    DNAzyme-dependent Analysis of rRNA 2’-O-Methylation09:12

    DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

    Published on: September 16, 2019

    Related Concept Videos

    RNA Splicing01:32

    RNA Splicing

    Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
    Bacterial RNA Polymerase00:43

    Bacterial RNA Polymerase

    Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
    In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
    RNA Splicing01:32

    RNA Splicing

    Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
    Bacterial Transcription01:53

    Bacterial Transcription

    RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
    Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
    Bacterial RNA Polymerase00:43

    Bacterial RNA Polymerase

    Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
    In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
    DNA Bacteriophages01:26

    DNA Bacteriophages

    Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...