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Bioluminescence assay of calcium with a liquid scintillation counter
Experientia
|August 15, 1983
Abstract:
The luminescence produced by Ca aequorin reaction in dilute solutions decays exponentially over a relatively prolonged period of time. The concentration of total Ca and of free Ca in Ca-EGTA buffers could be determined by measuring the decay of luminescence in a liquid scintillation counter. The method is also suitable for determining total Ca concentration in small tissue samples.