Permeabilization of Bacillus subtilis to chemotaxis methyltransferase II

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Published on: February 15, 2012

A Burgess-Cassler, G W Ordal

    Journal of Bacteriology
    |August 1, 1983
    PubMed

    Abstract:

    Treatment of Bacillus subtilis with 0.4% (vol/vol) toluene renders cells permeable not only to small molecules but also apparently to proteins as large as 30,000 daltons. Methyl-accepting chemotaxis proteins and two smaller polypeptides were methylated when B. subtilis methyltransferase II was added to permeabilized cells.

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