Restriction of developmental potential and trochoblast ciliation in Patella embryos

At the 64-cell-stage embryos of Patella develop a prototroch consisting of four groups of four cilia-bearing cells. Ciliogenesis of isolated blastomeres and trochoblasts was studied, as well as the effect on it of cleavage arrest caused by cytochalasin B treatment. Isolation of blastomeres or trochoblast cells has no influence on ciliogenesis; neither has arrest of cleavage in whole embryos after the third cleavage. However, cleavage arrest before third cleavage completely prevents ciliogenesis. Thus, third cleavage is decisive for the expression of the developmental potential of the primary trochoblasts. Impairment of DNA synthesis by aphidicolin in the S-phase preceding third cleavage also prevents ciliogenesis. It is concluded that a determinant for ciliogenesis as well as certain nuclear factors must be segregated into the micromeres at third cleavage for ciliogenesis to occur in the prototroch cells.