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Sequential histological changes and mast cell response in skin during chemically-induced carcinogenesis
Journal of Oral Pathology
|August 1, 1983
Summary
Dimethylbenzanthracene (DMBA) induces skin tumors with mast cell accumulation. This study models skin carcinogenesis, revealing mast cell roles in epithelial proliferation and providing a tool for mast cell kinetics research.
Area of Science:
- Oncology
- Dermatology
- Immunology
Background:
- Skin tumors induced by dimethylbenzanthracene (DMBA) are characterized by dense subepithelial mast cell accumulations.
- Understanding the dynamic changes in mast cell populations during carcinogenesis is crucial.
Purpose of the Study:
- To investigate sequential mast cell population changes during DMBA-induced skin carcinogenesis.
- To establish a model for studying mast cell proliferation and kinetics.
Main Methods:
- Swiss Webster mice were treated with DMBA in benzene applied to the back skin twice weekly for 12 weeks.
- Control and DMBA-treated tissues underwent histological examination to assess sequential tissue changes.
- Mast cell populations were quantified and analyzed in relation to epithelial alterations.
Main Results:
- Four distinct phases of tissue changes were observed: inflammation/necrosis, hyperplasia, disorganized growth with keratin pearls, and verrucous carcinoma-like nodules.
- Subepithelial mast cell numbers varied significantly throughout these phases, with dense foci noted beneath hyperplastic epithelium.
- A strong association was found between mast cell accumulation and abnormal epithelial proliferation.
Conclusions:
- Mast cells may contribute to abnormal epithelial proliferation during skin carcinogenesis.
- DMBA-induced skin tumors in mice offer a suitable model for investigating mast cell origin and kinetics.