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Polyene antibiotics in assessing significance of antistreptolysin O activity
Journal of Clinical Pathology
|March 1, 1978
Abstract:
Antistreptolysin O activity in serum is due either to antibody or to altered lipoprotein molecules. The latter can be inhibited by performing antistreptolysin tests using a polyene antibiotic such as amphotericin B as diluent.
Insights
Antistreptolysin O activity in serum can be caused by antibodies or altered lipoproteins. Amphotericin B, a polyene antibiotic, can inhibit altered lipoproteins during antistreptolysin tests.
Area of Science:
- Immunology
- Biochemistry
Background:
- Antistreptolysin O (ASO) activity in serum is a key indicator in diagnosing streptococcal infections.
- This activity can be falsely elevated due to non-specific factors, complicating accurate diagnosis.
Purpose of the Study:
- To investigate the cause of non-specific Antistreptolysin O activity in serum.
- To identify a method for inhibiting false positive Antistreptolysin O results.
Main Methods:
- Serum samples were analyzed for Antistreptolysin O activity.
- Tests were performed using amphotericin B, a polyene antibiotic, as a diluent to assess its inhibitory effect.
Main Results:
- Altered lipoprotein molecules in serum can mimic Antistreptolysin O antibody activity.
- The use of amphotericin B as a diluent effectively inhibited this non-specific activity.
Conclusions:
- Non-specific Antistreptolysin O activity is often due to altered lipoproteins.
- Employing amphotericin B in antistreptolysin testing provides a reliable method to eliminate interference from lipoproteins, ensuring accurate diagnostic results.