Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional identity between murine gamma interferon and macrophage activating factor.

R M Schultz, W J Kleinschmidt

    Nature
    |September 15, 1983
    PubMed
    Summary

    This study demonstrates that purified gamma interferon (IFN-γ) exhibits macrophage activating factor (MAF) activity, suggesting they are the same molecule. Further research is needed to confirm if IFN-γ accounts for all MAF activity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    HDAC1 and HDAC2 in mouse oocytes and preimplantation embryos: Specificity versus compensation.

    Cell death and differentiation·2016
    Same author

    BRD4 regulates Nanog expression in mouse embryonic stem cells and preimplantation embryos.

    Cell death and differentiation·2014
    Same author

    Behavior problems of handicapped adolescent female students.

    Journal of youth and adolescence·2013
    Same author

    Behavior problems of handicapped adolescent female students.

    Journal of youth and adolescence·2013
    Same author

    Biochemical studies of mammalian oogenesis: metabolic cooperativity between granulosa cells and growing mouse oocytes.

    Developmental biology·2010
    Same author

    Salmon poisoning disease in dogs: 29 cases.

    Journal of veterinary internal medicine·2010

    Area of Science:

    • Immunology
    • Molecular Biology
    • Cell Biology

    Background:

    • Macrophage activating factor (MAF) and gamma interferon (IFN-γ) share notable similarities in biosynthesis, inactivation, and purification.
    • Previous studies indicated potential identity between MAF and IFN-γ based on antibody neutralization and physicochemical properties.

    Purpose of the Study:

    • To definitively determine if gamma interferon (IFN-γ) is the molecule responsible for macrophage activating factor (MAF) activity.
    • To investigate the MAF activity of purified murine IFN-γ produced in a non-lymphoid cell line.

    Main Methods:

    • Cloning and expression of murine IFN-γ in a simian nonlymphoid cell line.
    • Assaying the purified, expressed IFN-γ for MAF activity.

    Main Results:

    Related Experiment Videos

    • Purified murine gamma interferon (IFN-γ), cloned and expressed in a simian cell line, demonstrated significant macrophage activating factor (MAF) activity.
    • These findings provide strong evidence for the identity of MAF and IFN-γ.

    Conclusions:

    • The results strongly support the hypothesis that gamma interferon (IFN-γ) is a key mediator of macrophage activation.
    • The potential for MAF heterogeneity in T cell-conditioned media warrants further investigation to fully elucidate the role of IFN-γ.