Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Some properties of mouse platelets.

W I Rosenblum, G H Nelson, C S Cockrell

    Thrombosis Research
    |May 15, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Gene discovery and gene function assignment in filamentous fungi.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    Traumatic injury of cultured astrocytes alters inositol (1,4,5)-trisphosphate-mediated signaling.

    Glia·2001
    Same author

    Traumatic injury of cortical neurons causes changes in intracellular calcium stores and capacitative calcium influx.

    The Journal of biological chemistry·2000
    Same author

    Astrocytes generate isoprostanes in response to trauma or oxygen radicals.

    Journal of neurotrauma·2000
    Same author

    Stretch-induced injury alters mitochondrial membrane potential and cellular ATP in cultured astrocytes and neurons.

    Journal of neurochemistry·2000
    Same author

    Alterations in calcium-mediated signal transduction after traumatic injury of cortical neurons.

    Cell calcium·2000

    Mouse platelets aggregate similarly to other mammals, responding to various agonists and undergoing the release reaction. Their pathways for arachidonate metabolism make them a valuable, cost-effective model for platelet aggregation studies.

    Area of Science:

    • Hematology
    • Pharmacology
    • Biomedical Research

    Background:

    • Platelet aggregation is crucial for hemostasis and thrombosis.
    • Understanding mammalian platelet function is vital for cardiovascular research.
    • The mouse is increasingly used as a model organism in biomedical studies.

    Purpose of the Study:

    • To characterize the aggregation and release reactions of mouse platelets.
    • To investigate the metabolic pathways involved in mouse platelet activation.
    • To evaluate the suitability of mouse platelets as a model for platelet research.

    Main Methods:

    • Platelet-rich plasma (PRP) from mice was prepared and tested for aggregation.
    • Aggregation was induced using agonists like arachidonate, thrombin, collagen, and ADP.

    Related Experiment Videos

  • The effects of cyclooxygenase inhibitors and prostacyclin on aggregation were assessed.
  • Metabolic studies utilized tritiated arachidonate to identify pathway activity.

    • Main Results:

    • Mouse platelets showed higher aggregation in heparinized PRP compared to citrated PRP.
    • Platelets underwent a release reaction with arachidonate, thrombin, and collagen, but not ADP.
    • Arachidonate-induced aggregation was inhibited by cyclooxygenase inhibitors and prostacyclin.
    • Mouse platelets possess both lipoxygenase and cyclooxygenase pathways, producing thromboxane and 12-HETE.

    Conclusions:

    • Mouse platelets exhibit aggregation and release characteristics similar to other mammals.
    • The identified metabolic pathways (cyclooxygenase and lipoxygenase) are conserved.
    • The mouse is a cost-effective and convenient model for studying platelet aggregation and related conditions.