[Molecular DNA-DNA hybridization in Pseudomonas fluorescens and Pseudomonas putida]

Taxonomic analysis was carried out and DNA-DNA homology studied in cultures of subgroups formed by numerical classification of 124 Pseudomonas fluorescens and P. putida strains. The GC content in the DNA of strains belonging to P. fluorescens subgroups varied from 61.2 to 64.5%, and their DNA-DNA homology with the type strain P. fluorescens ATCC 13525 was 24 to 83%. The lowest genome relatedness with the type culture was found in biotype C of P. fluorescens and P. aureofaciens strains. The GC content in the DNA of strains belonging to two subgroups formed by numerical classification of P. putida varied from 63.8 to 65.0%, and the homology of their DNA with the DNA of the type strain of P. fluorescens was 0 to 10% (for strains of the first and second subgroups, respectively).