[The effect of the method of adding ethylene glycol to turkey ejaculate on the quality of cryopreserved sperm]

Ethylene glycol at concentrations of 8 + 8, 4 + 12 or 0 + 16% was added to the ejaculate of the White Broad-Breasted turkey-stags by the method of (ejaculate : diluent) 1 : (1 + 1), 1 : (1 + 1/4 + 1/4 + 2/4) or 1 : (1 + 1/15 + 1/15 + 1/15 + 2/15 + + 4/15 + 6/15) at temperatures of 25, 15 or 5 degrees C. The dissolved ejaculate was equilibrated for 15 minutes, three minutes it was left to freeze in liquid nitrogen vapours and 24 hours it was stored at a temperature of -196 degrees C. Motility, percentage of live spermatozoa and percentage of morphologically normal spermatozoa were evaluated after sample thawing at 15 degrees C. The quality of cryo-preserved spermatozoa of stags was most effectively influenced by the addition of ethylene glycol to ejaculate at the concentration of 0 + 16% by the method of 1 : (1 + 1/15 + 1/15 + + 1/15 + 2/15 + 4/15 + 6/15) at the temperature of 25 degrees C.