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Related Experiment Videos

Human factor VIII: a calcium-linked protein complex.

M E Mikaelsson, N Forsman, U M Oswaldsson

    Blood
    |November 1, 1983
    PubMed
    Summary

    Calcium (Ca2+) is crucial for stabilizing human factor VIII coagulant activity (VIII:C). Maintaining physiologic calcium levels enhances factor VIII stability, preventing its deterioration in plasma.

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    Isolation and characterization of human factor VIII: molecular forms in commercial factor VIII concentrate, cryoprecipitate, and plasma.

    Proceedings of the National Academy of Sciences of the United States of America·1986
    See all related articles

    Area of Science:

    • Biochemistry
    • Hematology

    Background:

    • Factor VIII is essential for blood coagulation.
    • The role of calcium in factor VIII complex stability is not fully understood.

    Purpose of the Study:

    • To investigate the role of calcium (Ca2+) in the human factor VIII complex.
    • To determine how calcium influences factor VIII coagulant activity (VIII:C) stability.

    Main Methods:

    • Stability studies of factor VIII in plasma under various conditions.
    • Metal determination using atomic absorption spectrophotometry.
    • Gel filtration chromatography of factor VIII complex with and without EDTA.

    Main Results:

    • Factor VIII coagulant activity (VIII:C) deteriorates in citrated plasma but is stabilized by heparin or calcium chloride (CaCl2).
    • VIII:C is stable at physiologic ionized calcium levels.
    • Purified factor VIII contains intrinsic calcium, removable by EDTA, which dissociates factor VIII antigens.

    Conclusions:

    • Human factor VIII circulates in plasma as a calcium-linked protein complex.
    • Calcium is essential for maintaining the structural integrity and activity of the factor VIII complex.

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