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DNA interference with radioimmunoassay procedures.

N Matsuo, S S Koide

    Clinical Chemistry
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    A bacterial factor, identified as deoxyribonucleic acid (DNA), was isolated from Streptococcus faecalis. This DNA prevents immune complex precipitation, potentially causing false-positive results in radioimmunoassay (RIA) procedures.

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    Area of Science:

    • Microbiology
    • Immunology
    • Biochemistry

    Background:

    • Immune complex precipitation is a key step in many immunoassays.
    • Bacterial components can potentially interfere with immunological assays.
    • Streptococcus faecalis is a common bacterium with various biological activities.

    Purpose of the Study:

    • To identify and characterize a bacterial factor that prevents immune complex precipitation.
    • To investigate the mechanism by which this factor interferes with immunoassays.
    • To assess the implications of this interference for radioimmunoassay (RIA) procedures.

    Main Methods:

    • Purification of the bacterial factor using gel filtration chromatography (Sephadex G-100, Bio-Gel A-150m).
    • Identification of the factor using ultraviolet absorption spectroscopy, deoxyribonuclease hydrolysis, molecular weight estimation, and chemical assays (diphenylamine).

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  • Testing the effect of the purified factor on immune complex precipitation and radioimmunoassay (RIA) protocols.

    • Main Results:

    • A factor preventing immune complex precipitation was isolated from Streptococcus faecalis.
    • The factor was identified as deoxyribonucleic acid (DNA) based on its biochemical properties.
    • Double-stranded DNA and polynucleotides were found to inhibit immune complex precipitation.
    • This DNA interference can lead to false-positive results in standard RIA procedures.

    Conclusions:

    • Deoxyribonucleic acid (DNA) from Streptococcus faecalis acts as an inhibitor of immune complex precipitation.
    • The presence of double-stranded DNA can compromise the accuracy of radioimmunoassays (RIA) by causing false-positive interference.
    • Careful consideration of potential DNA contamination is necessary when performing RIA.