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An in vitro system for measuring endothelial permeability under hydrostatic pressure
Experimental Cell Research
|October 15, 1983
Summary
This study shows that endothelial cell permeability to fluid is pressure-dependent and requires calcium ions (Ca2+). Reduced Ca2+ increases flow, while its reintroduction decreases it, highlighting Ca2+'s role in regulating endothelial barrier function.
Area of Science:
- Endothelial cell biology
- Vascular physiology
- Biophysics
Background:
- Endothelial cells form a barrier regulating fluid and solute transport.
- Understanding factors influencing endothelial permeability is crucial for vascular health.
Purpose of the Study:
- To develop a system for measuring endothelial permeability under controlled pressure gradients.
- To investigate the role of hydrostatic pressure and calcium ions (Ca2+) in regulating endothelial permeability.
Main Methods:
- Cultured porcine aortic endothelial cells on microporous substrata in a two-compartment chamber.
- Applied transendothelial pressure gradients and measured fluid flow rates.
- Manipulated extracellular Ca2+ concentrations using EGTA and Ca2+ readdition.
Main Results:
- Hydrostatic pressure gradients induced a pressure-dependent decrease in fluid flow, reaching steady state.
- Decreased endothelial permeability was observed only in the presence of Ca2+.
- EGTA-induced Ca2+ removal increased flow, while Ca2+ readdition decreased it in a concentration-dependent manner.
Conclusions:
- Endothelial permeability is significantly influenced by hydrostatic pressure.
- Calcium ions (Ca2+) are essential for pressure-induced changes in endothelial permeability.
- The developed system effectively models and quantifies endothelial barrier function.