High-performance liquid chromatography of staphylococcal enterotoxin B
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Updated: Oct 13, 2026

Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
Published on: October 1, 2012
Abstract:
Size-exclusion, ion-exchange, and reversed-phase chromatography were investigated for use in purification and characterization of Staphylococcal enterotoxin B (SEB), a 28,000 Mr protein associated with Staphylococcal food-borne intoxication. In all approaches chromatography of crude or purified SEB yielded one or more components which displayed toxin activity and contained the 28,000 Mr SEB protein plus other lower-molecular-weight protein species. Reversed-phase chromatography of detergent-treated SEB permitted resolution of the 28,000 Mr protein from lower-molecular-weight components.
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