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Macrophage activation by Lactobacillus casei in mice
Microbiology and Immunology
|January 1, 1983
Summary
Lactobacillus casei YIT 9018 (LC 9018) enhances macrophage functions, including phagocytic activity and acid phosphatase levels. This probiotic demonstrates potential for activating immune cells, suggesting therapeutic applications in cancer immunotherapy.
Area of Science:
- Immunology
- Microbiology
- Probiotics
Background:
- Lactobacillus casei YIT 9018 (LC 9018) exhibits antitumor properties against various murine tumors.
- Macrophage activation is a critical component of the immune response against cancer.
Purpose of the Study:
- To investigate the effects of LC 9018 on macrophage functions.
- To determine if LC 9018 can enhance immune cell activity relevant to antitumor responses.
Main Methods:
- Mice were injected intraperitoneally (i.p.) with LC 9018.
- Peritoneal macrophages were analyzed for acid phosphatase and phagocytic activity.
- Carbon clearance tests assessed reticuloendothelial system function.
- Cytostatic activity of peritoneal exudate cells (PEC) against tumor cells was measured.
Main Results:
- LC 9018 significantly enhanced acid phosphatase and phagocytic activity of peritoneal macrophages.
- Peak phagocytic activity occurred 2-3 days post-injection.
- LC 9018 accelerated carbon clearance, indicating enhanced reticuloendothelial system function.
- PEC induced by LC 9018 displayed enhanced cytostatic activity against EL4 tumor cells, unlike those induced by L. fermentum YIT 0159.
Conclusions:
- Lactobacillus casei YIT 9018 effectively activates macrophages in mice.
- LC 9018 demonstrates immunomodulatory effects, enhancing key macrophage functions.
- These findings support the potential of LC 9018 as an immunotherapeutic agent for cancer.