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Related Experiment Videos

[Immunochemical determination of human lactoferrin].

G P German, N V Lavrova, L A Sherer

    Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
    |September 1, 1983
    PubMed
    Summary

    Lactoferrin levels in human breast milk decrease after the first week of lactation. Microbial contamination may cause lactoferrin content to increase, regardless of lactation stage.

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    Chimeric lactase capable of spontaneous and strong immobilization on cellulose and development of a continuous-flow system for lactose hydrolysis at high temperatures.

    Applied and environmental microbiology·2010

    Area of Science:

    • Biochemistry
    • Immunology
    • Human Physiology

    Context:

    • Lactoferrin, an iron-binding protein, plays a crucial role in the innate immune system of human milk.
    • Quantifying lactoferrin is essential for understanding its role in infant nutrition and protection.
    • Accurate measurement methods are needed to study lactoferrin dynamics during lactation.

    Purpose:

    • To develop and validate a method for quantifying lactoferrin in human milk.
    • To investigate the changes in lactoferrin concentration during the lactation period.
    • To explore the relationship between microbial contamination and lactoferrin levels in human milk.

    Summary:

    • Immunochemically pure lactoferrin was isolated from human colostrum for antiserum production and as a reference standard.
    • Monospecific antiserum was generated and utilized in radial immunodiffusion assays to determine lactoferrin content in human milk samples.
    • Lactoferrin levels exhibited significant fluctuations, notably decreasing by the second week postpartum. An increase in lactoferrin was observed in cases of microbial contamination.

    Impact:

    • Establishes a reliable method for lactoferrin quantification in human milk.
    • Provides insights into the dynamic changes of lactoferrin during lactation.
    • Suggests lactoferrin may serve as an indicator of microbial contamination in human milk, potentially impacting infant health.

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