Basic fuchsins and the Schiff-aldehyde reaction. I. Spectral absorption characteristics in solution

Spectral absorption profiles at the UV and visible ranges were detected spectrophotometrically for solutions of various basic fuchsins, for the SCHIFF reagent prepared from these stains and for the end product from SCHIFF-formaldehyde reaction. The aim was to detect whether absorption spectra could vary as a function of the basic fuchsin origin up to the point of being suspected of affecting potentially the spectrum of FEULGEN-stained chromatin. The results indicate a constancy in the spectral absorption characteristics for the absorption profiles at the visible range, when one considers SCHIFF reagents prepared from various basic fuchsins added with aldehyde. However, it is possible that size, stereoarrangement and electron distribution of the aldehyde- (eventually, ketone-) bearing molecule involved in SCHIFF-carbonyl reactions would function as auxochroms, the spectral characteristics of which could be affected by dye content and contaminants of the basic fuchsin.