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Related Experiment Videos

Disaccharidases in coeliac disease.

K Horváth, G Horn, H Bodánszky

    Acta Paediatrica Hungarica
    |January 1, 1983
    PubMed
    Summary

    In pediatric malabsorption cases, jejunal biopsy revealed decreased disaccharidase activity, particularly lactase, in most coeliac disease diagnoses. Routine enzyme testing is not essential for diagnosing coeliac disease.

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    Area of Science:

    • Pediatric Gastroenterology
    • Gastrointestinal Physiology

    Background:

    • Malabsorption in children presents with varied symptoms.
    • Congenital enzyme deficiencies are a consideration but often excluded.
    • Coeliac disease is a common cause of pediatric malabsorption.

    Purpose of the Study:

    • To investigate disaccharidase enzyme activities in children with malabsorption symptoms.
    • To correlate enzyme activity with histological findings in jejunal biopsies.
    • To assess the diagnostic utility of disaccharidase assays in coeliac disease.

    Main Methods:

    • Jejunal biopsies were obtained from 30 children with malabsorption symptoms.
    • Histology was performed to assess villous atrophy.
    • Activities of lactase, saccharase, and maltase were determined in tissue samples.

    Main Results:

    • Coeliac disease was confirmed in 23 out of 30 cases.
    • Decreased disaccharidase activity was observed in cases with villous atrophy.
    • Lactase activity was most frequently reduced (69% absent), followed by saccharase (29%) and maltase (4%).
    • No strong correlation was found between the degree of villous atrophy and enzyme activity levels.

    Conclusions:

    • Reduced disaccharidase activity indicates impaired disaccharide splitting capacity, not necessarily symptomatic malabsorption.
    • Routine determination of disaccharidase activities is not recommended for diagnosing coeliac disease.

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