Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Are blastocyst prostaglandins produced endogenously?

C Racowsky, J D Biggers

    Biology of Reproduction
    |September 1, 1983
    PubMed
    Summary

    Preimplantation blastocysts metabolize arachidonic acid (AA) differently. Rabbit blastocysts produce prostaglandins (PGs) and thromboxane B2, while mouse blastocysts do not, suggesting species-specific roles in implantation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Metabolic imaging of human cumulus cells reveals associations with pregnancy and live birth.

    Human reproduction (Oxford, England)·2024
    Same author

    Recombinant human chorionic gonadotropin and gonadotropin-releasing hormone agonist differently affect the profile of extracellular vesicle microRNAs in human follicular fluid.

    Journal of assisted reproduction and genetics·2023
    Same author

    Embryo attrition in planned PGT-A: predicting the number of available blastocysts for transfer.

    Journal of assisted reproduction and genetics·2022
    Same author

    Blastocyst conversion rate and ploidy in patients with structural rearrangements.

    Journal of assisted reproduction and genetics·2021
    Same author

    Responses to fertility treatment among patients with cancer: a retrospective cohort study.

    Fertility research and practice·2018
    Same author

    The effect of short-term exposure of cumulus-oocyte complexes to in vitro maturation medium on yield of mature oocytes and usable embryos in stimulated cycles.

    Journal of assisted reproduction and genetics·2018

    Area of Science:

    • Reproductive Biology
    • Biochemistry

    Background:

    • Preimplantation embryos utilize various metabolic pathways.
    • Prostaglandins (PGs) are implicated in implantation, but their source in early embryos is unclear.

    Purpose of the Study:

    • To investigate cyclooxygenase activity in mouse and rabbit blastocysts.
    • To determine if blastocysts metabolize arachidonic acid (AA) to prostaglandins (PGs) and thromboxane B2.

    Main Methods:

    • Incubation of mouse and rabbit blastocysts with radiolabeled arachidonic acid (AA).
    • Stimulation of AA release using a calcium ionophore (X-537A).
    • Analysis of AA metabolism using thin-layer chromatography and liquid scintillation spectroscopy.

    Main Results:

    • Both mouse and rabbit blastocysts incorporated AA into phospholipids and neutral lipids.
    • Rabbit blastocysts metabolized AA to various PGs and thromboxane B2 in incubation media.
    • Mouse blastocysts showed no detectable PG synthesis after ionophore stimulation.

    Conclusions:

    • Rabbit blastocysts possess cyclooxygenase activity, producing PGs and thromboxane B2.
    • Mouse blastocysts lack detectable cyclooxygenase activity at this stage.
    • Blastocyst-derived PGs may play a species-specific role in implantation.

    Related Experiment Videos