Specific effect of dietary saturated fat on rat aortic 6-keto-PGF1 alpha synthesis

The effect of dietary lard and corn oil-supplementation on rat aortic 6-keto-PGF1 alpha synthesis from both exogenous and endogenous arachidonic acid was investigated. Only lard-supplementation resulted in any change when compared to rats fed normal chow. Lard-supplementation resulted in both a significant increase in the conversion of exogenous arachidonic acid to 6-keto-PGF1 alpha and a significant decrease in the ratio of linoleate to arachidonate in aortic phospholipids. Basal aortic synthesis of 6-keto-PGF1 alpha from endogenous arachidonic acid was not different for any group. We conclude that at least for the aortic conversion of exogenous arachidonic acid to 6-keto-PGF1 alpha the level of inhibitor linoleic acid in tissue phospholipids may be important.