Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural correlates of immunoglobulin diversity.

M Potter

    Survey of Immunologic Research
    |January 1, 1983
    PubMed
    Summary

    The variable domains of antibodies, despite diverse structures, share a conserved framework. Antigen-binding sites are primarily located on variable complementarity-determining regions, formed by gene segments, contributing to antibody diversity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Smoking and drinking habits of women in subsequent pregnancies after specific advice about the dangers of these exposures during pregnancy.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2021
    Same author

    Restarting plastic surgery: Drawing on the experience of the initial COVID-19 pandemic to inform the safe resumption of services.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2020
    Same author

    Comparison of sporadic and familial behavioral variant frontotemporal dementia (FTD) in a North American cohort.

    Alzheimer's & dementia : the journal of the Alzheimer's Association·2020
    Same author

    Microbial Hazards and Emerging Issues Associated with Produce <sup>†</sup> A Preliminary Report to the National Advisory Committee on Microbiologic Criteria for Foods.

    Journal of food protection·2019
    Same author

    EBMT prospective observational study on allogeneic hematopoietic stem cell transplantation in T-prolymphocytic leukemia (T-PLL).

    Bone marrow transplantation·2019
    Same author

    Adverse obstetric history is not a risk factor for poor outcome after ventral rectopexy for obstructive defaecation syndrome.

    Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2018

    Area of Science:

    • Immunology and Molecular Biology: Focuses on antibody structure-function relationships and genetic mechanisms of diversity.

    Background:

    • Antibody variable (FV) regions, composed of variable light (VL) and variable heavy (VH) domains, exhibit conserved framework structures across species.
    • The FV region's surface features highly variable polypeptide segments, known as complementarity-determining regions (CDRs), which are crucial for antigen recognition.

    Purpose of the Study:

    • To elucidate the structural basis of antibody-antigen interactions and the contribution of various gene segments to antibody diversity.
    • To investigate the role of conserved framework structures and variable CDRs in forming antigen-binding sites.

    Main Methods:

    • Analysis of primary protein structures and interactions between VL and VH domains.
    • Identification of polypeptide segments derived from VL, VH, JH, and D gene products forming the FV surface.
    • Review of immunochemical data and amino acid contact identification.

    Main Results:

    • A conserved framework structure forms the bulk of the FV region, with variable CDRs constituting the antigen-binding surface.
    • VL and VH gene products, along with segments from JH and D genes, contribute to the structural diversity and antigen-reactive surface.
    • While CDRs are implicated in antigen binding, the precise roles of VHD-JH junctions and D gene products are still under investigation.

    Conclusions:

    • The combination of VL and VH gene products is a major driver of antibody structural diversity.
    • Gene rearrangement and somatic mutations further amplify antibody diversity, with conserved frameworks providing structural stability.
    • Further research is needed to fully understand the contribution of gene rearrangement processes, particularly VHD-JH, to antibody diversity and antigen binding.

    Related Experiment Videos