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Fibroblastoid colony-forming cells in myeloproliferative disorders
Abstract:
The properties of human bone marrow fibroblastoid colonies (CFU-F) were studied in normal subjects and in patients with myeloproliferative disorders. Colony incidence was within normal values in all groups of patients analyzed except for myelodisplastic syndromes, with higher mean value. The growth rate of CFU-F is inversely related to the initial colony-forming efficiency both in normal subjects and in patients. Direct correlation between CFU-F and granulocyte-macrophage colony-forming unit (GM-CFU) was detected only in normal subjects, but lacked in patients. Higher number of CFU-F was observed in subjects with increased incidence of bone marrow megakaryocytes or peripheral blood platelets, irrespective of the underlying disorders and the platelet-derived growth-factorenhanced cloning efficiency of bone marrow fibroblasts.
Insights
Human bone marrow fibroblastoid colony (CFU-F) incidence is normal in most myeloproliferative disorders but elevated in myelodysplastic syndromes. CFU-F growth correlates inversely with colony-forming efficiency and is linked to megakaryocytes and platelets.
Area of Science:
- Hematology
- Stem Cell Biology
- Oncology
Background:
- Fibroblastoid colonies from human bone marrow (CFU-F) are crucial for hematopoiesis.
- Their role in myeloproliferative disorders (MPDs) requires further elucidation.
- Understanding CFU-F properties can offer insights into bone marrow microenvironment dysregulation.
Purpose of the Study:
- To investigate the characteristics of CFU-F in normal individuals and patients with MPDs.
- To determine the relationship between CFU-F and other hematopoietic progenitor cells (e.g., GM-CFU).
- To explore the influence of megakaryocytes and platelets on CFU-F behavior.
Main Methods:
- Colony-forming unit-fibroblastoid (CFU-F) assays were performed on bone marrow samples.
- Colony incidence and growth rates were analyzed.
- Correlations between CFU-F, granulocyte-macrophage colony-forming units (GM-CFU), megakaryocytes, and platelets were assessed.
Main Results:
- CFU-F incidence was normal in most MPDs but higher in myelodysplastic syndromes.
- CFU-F growth rate showed an inverse relationship with initial colony-forming efficiency in all subjects.
- A direct correlation between CFU-F and GM-CFU was observed only in normal subjects, not in patients.
- Increased CFU-F numbers were associated with higher megakaryocyte and platelet counts, independent of the disorder.
Conclusions:
- CFU-F exhibit altered incidence in specific bone marrow disorders like myelodysplastic syndromes.
- The relationship between CFU-F and GM-CFU is disrupted in MPDs.
- Bone marrow fibroblasts and megakaryocyte/platelet lineages may interact, influencing CFU-F behavior in MPDs.