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Updated: Aug 1, 2026

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method
Published on: April 18, 2019
In vitro effect of combinations of antimicrobial agents and EDTA-tromethamine on certain gram-positive bacteria
Abstract:
Combinations of EDTA-tromethamine and 7 antimicrobial agents (chloramphenicol, nalidixic acid, oxytetracycline, penicillin, polymyxin-B, streptomycin, and a triple sulfonamide preparation) were tested for synergistic activities against Staphylococcus aureus, Corynebacterium renal, Listeria monocytogenes, Erysipelothrix rhusiopathiae, and a beta-hemolytic streptococci. Two in vitro tests were used--minimal inhibitory concentrations of the drugs and a 2-dimensional Microtiter checkerboard technique. A slight synergistic action was seen when L monocytogenes was exposed to combinations of penicillin-EDTA or streptomycin-EDTA.
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