Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in membrane phospholipid distribution during platelet activation.

E M Bevers, P Comfurius, R F Zwaal

    Biochimica Et Biophysica Acta
    |December 7, 1983
    PubMed
    Summary

    Platelet activation by collagen plus thrombin, diamide, or ionophore exposes phosphatidylserine on the outer surface. This phospholipid shift enhances thrombin generation, crucial for hemostasis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Dissociation of yeast ribosomes; The effects of hydrostatic pressure and KCl.

    Molecular biology reports·2013
    Same author

    Calcium-activated and apoptotic phospholipid scrambling induced by Ano6 can occur independently of Ano6 ion currents.

    Cell death & disease·2013
    Same author

    Direct inhibition of phospholipid scrambling activity in erythrocytes by potassium ions.

    Cellular and molecular life sciences : CMLS·2008
    Same author

    Microparticles adhere to collagen type I, fibrinogen, von Willebrand factor and surface immobilised platelets at physiological shear rates.

    British journal of haematology·2007
    Same author

    Anticoagulant and antithrombotic properties of intracellular protease-activated receptor antagonists.

    Journal of thrombosis and haemostasis : JTH·2006
    Same author

    Activated scramblase and inhibited aminophospholipid translocase cause phosphatidylserine exposure in a distinct platelet fraction.

    Cellular and molecular life sciences : CMLS·2005

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Hematology

    Background:

    • Platelet surface phospholipids play a role in hemostasis.
    • Understanding phospholipid exposure during platelet activation is key.

    Purpose of the Study:

    • To investigate phospholipid exposure on activated platelets.
    • To determine the functional consequences of altered phospholipid orientation.

    Main Methods:

    • Incubation of platelets with phospholipase A2 and sphingomyelinase.
    • Activation of platelets using collagen, thrombin, diamide, or calcium ionophore.
    • Assessing phospholipid exposure and prothrombinase activity.

    Main Results:

    • Platelets activated by collagen plus thrombin, diamide, or ionophore showed increased phosphatidylserine exposure.
    • Sphingomyelin exposure decreased on activated platelets.
    • Activated platelets exhibited enhanced prothrombin to thrombin conversion.

    Conclusions:

    • Platelet activation causes transbilayer phospholipid movement.
    • Increased phosphatidylserine exposure on activated platelets is important for hemostasis.

    Related Experiment Videos