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B cell maturation factor: effects on various cell populations.

C L Sidman, J D Marshall

    Journal of Immunology (Baltimore, Md. : 1950)
    |February 1, 1984
    PubMed
    Summary
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    B cell maturation factor (BMF) stimulates B cells to secrete immunoglobulin (Ig). This lymphokine enhances Ig heavy and light chain synthesis and alters secretory to membrane forms, even after short exposure, leading to B cell death.

    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • B cell maturation factor (BMF) is a lymphokine involved in B lymphocyte maturation.
    • Understanding BMF's role in immunoglobulin (Ig) secretion is crucial for B cell biology.

    Purpose of the Study:

    • To investigate the mechanism by which B cell maturation factor (BMF) induces immunoglobulin (Ig) secretion in B cells.
    • To analyze the kinetics and molecular events underlying BMF-mediated B cell activation.

    Main Methods:

    • Utilized murine splenic B lymphocytes and the WEHI-279 B cell line.
    • Employed fluorescence-activated cell sorting (FACS) for B cell purification.
    • Performed limiting dilution cultures to assess BMF responsiveness.

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    Main Results:

    • BMF directly induces Ig secretion in various B cell subsets, independent of T cells, LPS receptors, or MHC haplotype.
    • The primary mechanism involves enhanced, balanced synthesis of Ig heavy (H) and light (L) chains.
    • BMF also increases the ratio of secretory to membrane H chains, preceding increased H and L chain synthesis, and induces B cell death.

    Conclusions:

    • BMF is a potent inducer of Ig secretion in B cells, acting directly on these cells.
    • BMF orchestrates a complex response including altered Ig chain production, kinetics, and cell fate.
    • BMF can program significant B cell responses rapidly, despite the multi-day timeline for full molecular and cellular changes.