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Isolation and characterization of pock-forming plasmids for Streptomyces griseus from soil actinomycetes

T Ohnuki, T Imanaka, S Aiba

    Gene
    |November 1, 1983
    PubMed

    Insights

    Researchers isolated six novel pock-forming plasmids from soil actinomycetes. These plasmids, capable of inhibiting sporulation, were characterized for their genetic properties and stability in Streptomyces griseus.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Actinomycetes are soil-dwelling bacteria known for producing antibiotics.
    • Plasmids are extrachromosomal DNA elements that can confer advantageous traits to bacteria.
    • Understanding plasmid biology is crucial for genetic engineering and antibiotic production.

    Purpose of the Study:

    • To isolate and characterize novel plasmids from soil actinomycetes.
    • To identify plasmids that induce "pocks" (sporulation inhibition zones).
    • To analyze the genetic features and stability of these pock-forming plasmids.

    Main Methods:

    • Isolation of actinomycetes strains from soil samples.
    • Purification of plasmid DNA using CsCl-EtBr equilibrium density-gradient centrifugation.
    • Selection of pock-forming plasmids via protoplast transformation of Streptomyces griseus ATCC10137.

    Main Results:

    • Thirty independent actinomycetes strains carrying plasmids were identified.
    • Six pock-forming plasmids (pOA7, pOA11, pOA15, pOA23, pOA29, pOA30) were successfully isolated.
    • Cleavage maps, transformation frequencies, copy numbers, and stability of the selected plasmids were determined.

    Conclusions:

    • Novel pock-forming plasmids were successfully isolated from soil actinomycetes.
    • These plasmids exhibit varying genetic characteristics and stability.
    • The identified plasmids represent valuable tools for further research in Streptomyces genetics and antibiotic development.

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