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Serotypes of Pseudomonas aeruginosa isolated from laboratory rats and mice
Abstract:
Pseudomonas aeruginosa isolates cultured from the feces of laboratory rats and mice were serotyped. The fecal samples originated from primary genetic centers, secondary breeding facilities, and research testing facilities operated under contracts from the National Cancer Institute. Eighty-nine percent or 264 of 297 isolates were of serotypes 1, 4, 6, 10, or 11, and of these, 154 (51.8%) isolates were serotypes 6 or 11. In some instances, Pseudomonas aeruginosa serotypes found in animals at a primary genetic center were also found at secondary breeding facilities which had received breeding stock from the primary genetic center. The same serotypes also were found in animals at research-testing laboratories that had received animals from the secondary breeding facilities.
Insights
Common Pseudomonas aeruginosa serotypes were identified in laboratory rodents. Serotypes 6 and 11 were most prevalent, indicating potential transmission across animal breeding and research facilities.
Area of Science:
- Microbiology
- Veterinary Science
- Animal Health
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen frequently found in animal models.
- Understanding the serotype distribution in laboratory animals is crucial for experimental consistency and animal welfare.
- Rodent models are widely used in biomedical research, including studies funded by the National Cancer Institute.
Purpose of the Study:
- To serotype Pseudomonas aeruginosa isolates from the feces of laboratory rats and mice.
- To identify the predominant serotypes in animals sourced from different types of facilities.
- To investigate the potential transmission of specific serotypes within the animal supply chain.
Main Methods:
- Fecal samples were collected from laboratory rats and mice.
- Pseudomonas aeruginosa isolates were cultured and serotyped.
- Isolates were sourced from primary genetic centers, secondary breeding facilities, and research testing facilities.
Main Results:
- 297 Pseudomonas aeruginosa isolates were analyzed.
- 89% (264/297) of isolates belonged to serotypes 1, 4, 6, 10, or 11.
- Serotypes 6 and 11 accounted for 51.8% (154/297) of the isolates.
- Identical serotypes were found in animals across successive stages of the supply chain (primary, secondary, and research facilities).
Conclusions:
- Specific Pseudomonas aeruginosa serotypes, particularly 6 and 11, are common in laboratory rodents.
- The findings suggest a potential for serotype transmission between interconnected animal facilities.
- Monitoring and controlling P. aeruginosa serotypes in laboratory animal populations may be important for research integrity.