[Immunohistological detection of T lymphocytes with monoclonal antibody BL-T2]

Using the monoclonal Pan-T cell antibody BL-T2 T lymphocytes are identified on tissue sections with an indirect immunofluorescence technique. The T cell areas between the lymphoid follicles (B cells) are stained on cryostat sections of human palatine tonsils. A conventional heterologous anti-Ig antiserum was used for the detection of B lymphocytes. The identification of T and B lymphocytes by immunofluorescence allows statements about the distribution of both populations in tissue sections.