[Transducing activity of the temperate SM bacteriophage of Pseudomonas aeruginosa]

The temperate bacteriophage SM is not serologically related to the known transducing phages F116, G101, B3 of Pseudomonas aeruginosa. The strains with auxotrophic mutations within the wide ranges of the genetic map of P. aeruginosa strain PAO1 were used for studying the transducing activity of the SM phage. All of the 7 bacterial markers tested are transduced with SM phage grown on a prototrophic donor strain. The frequency of transduction of separate bacterial markers using the wild type SM phage is 2.3 to 4.6 X 10(-8). Linked ilv202+ - met28+ markers are cotransduced with SM phage at a frequency of about 1.5%.