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Core substructure in cyanobacterial phycobilisomes.

J C Gingrich, D J Lundell, A N Glazer

Journal of Cellular Biochemistry

|January 1, 1983

Summary

The tricylindrical core of Synechocystis 6701 phycobilisomes comprises four allophycocyanin complexes. Complexes C and D function as terminal energy acceptors, with a proposed model for the core structure.

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Phycobilisomes: macromolecular structure and energy flow dynamics.

Biophysical journal·2009

Area of Science:

* Photosynthesis research
* Molecular and structural biology

Background:

* Phycobilisomes are light-harvesting antenna complexes crucial for photosynthesis in cyanobacteria and algae.
* The core structure of phycobilisomes plays a vital role in efficient energy transfer to reaction centers.
* Synechocystis 6701 phycobilisomes possess a unique tricylindrical core structure.

Purpose of the Study:

* To elucidate the composition and structural organization of the tricylindrical core of Synechocystis 6701 phycobilisomes.
* To identify novel protein subunits and their roles within the phycobilisome core.
* To propose a model for the arrangement of core complexes based on biochemical analysis.

Main Methods:

* Biochemical analysis of phycobilisome complexes from Synechocystis 6701.
* Characterization of allophycocyanin-containing complexes and associated polypeptides.
* Spectroscopic analysis to determine the function of different complexes.

Main Results:

* Identified four types of allophycocyanin-containing complexes (A, B, C, D) in the tricylindrical core.
* Discovered a novel 18.5K polypeptide, a biliprotein subunit with a phycocyanobilin prosthetic group.
* Determined the molar ratio of complexes A:B:C:D as 4:4:2:2 and identified complexes C and D as terminal energy acceptors.

Conclusions:

* The tricylindrical core of Synechocystis 6701 phycobilisomes is composed of 12 subcomplexes.
* A model is proposed where the two proximal cylinders have an ABCD composition, and the distal cylinder has an A2B2 composition.
* The 18.5K polypeptide represents a significant new component in the phycobilisome energy transfer pathway.

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