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Updated: Jul 11, 2026

An Assay for Measuring the Activity of Escherichia coli Inducible Lysine Decarboxyase
Published on: December 20, 2010
[Cyanide-resistant oxygen consumption of the lysine-synthesizing bacteria Brevibacterium flavum 22 LD]
Abstract:
The growth of the culture and biosynthesis of lysin were studied in Brevibacterium flavum 22 LD cultivated in a chemostat. During cultivation the flow rate of the medium and the partial pressure of oxygen dissolved in the medium were varied. The maximum yield of lysine, calculated in respect to the sucrose consumed, (Yp = g lysine . HCl/g sucrose) was registered when cyanide-resistant oxygen consumption was the least. A change of the cultivation conditions provoked a decrease of Yp value and a simultaneous increase in cyanide-resistant respiration. Possible reasons of the phenomena observed are discussed.
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