Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Responses to thyrotropin during development in Japanese quail.

F M McNabb, F W Stanton, R T Weirich

    Endocrinology
    |April 1, 1984
    PubMed
    Summary

    Thyroid-pituitary feedback matures early in Japanese quail development. Posthatching, thyroid hormone responses to TSH change, suggesting peripheral conversion of thyroxine (T4) to triiodothyronine (T3) drives perinatal hormonal shifts.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Think before you prescribe: how dentistry contributes to antibiotic resistance.

    Australian dental journal·2019
    Same author

    Long- and Short-Range Electrostatic Fields in GFP Mutants: Implications for Spectral Tuning.

    Scientific reports·2015
    Same author

    Efficacy and safety of beloranib for weight loss in obese adults: a randomized controlled trial.

    Diabetes, obesity & metabolism·2015
    Same author

    The dentition: the outcomes of morphogenesis leading to variations of tooth number, size and shape.

    Australian dental journal·2014
    Same author

    General and craniofacial development are complex adaptive processes influenced by diversity.

    Australian dental journal·2014
    Same author

    Epigenetics: a new frontier in dentistry.

    Australian dental journal·2014

    Area of Science:

    • Endocrinology
    • Developmental Biology
    • Comparative Physiology

    Background:

    • The ontogeny of thyroid hormone regulation is crucial for development.
    • Japanese quail (Coturnix japonica) exhibit precocial development, making them a suitable model for studying embryonic thyroid function.
    • Understanding thyroid-pituitary feedback mechanisms and responses to thyroid-stimulating hormone (TSH) is key to deciphering developmental endocrinology.

    Purpose of the Study:

    • To investigate the developmental maturation of thyroid responses to TSH in Japanese quail embryos and chicks.
    • To determine the timing of thyroid-pituitary negative feedback maturation during embryonic development.
    • To characterize developmental changes in serum thyroid hormone (T3 and T4) levels and ratios following TSH administration.

    Main Methods:

    • Administration of thiourea to quail embryos to assess thyroid stimulation and feedback maturation.
    • Measurement of thyroid 32P uptake, serum T3 and T4 concentrations, thyroid weights, and body weights.
    • Intravenous administration of TSH to embryos and chicks at various developmental stages to evaluate dose- and time-dependent responses.

    Main Results:

    • Thyroid-pituitary negative feedback matures between days 9 and 10 of the 16.5-day incubation period.
    • Embryonic TSH administration leads to parallel increases in serum T3 and T4 without altering the T3:T4 ratio.
    • Posthatching TSH administration results in increased T4 but decreased T3:T4 ratios, indicating developmental shifts in hormonal response and peripheral conversion.

    Conclusions:

    • Hypothalamic or higher brain control appears to dominate pituitary regulation of the thyroid in late-term quail embryos.
    • Developmental changes in the hormonal response to TSH occur, with peripheral conversion of T4 to T3 likely accounting for the perinatal increase in the T3:T4 ratio.
    • TSH-mediated thyroid effects alone do not explain the perinatal surge in the T3:T4 ratio, highlighting the importance of peripheral deiodination.

    Related Experiment Videos