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Murine B cell differentiation lineages.

R R Hardy, K Hayakawa, D R Parks

    The Journal of Experimental Medicine
    |April 1, 1984
    PubMed
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    Two B cell lineages are distinguished by the expression patterns of BLA-1 and BLA-2 antigens during differentiation. B cell activation leads to the reappearance of these antigens, impacting antibody production.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • B cells express distinct cell surface antigens, BLA-1 and BLA-2, recognized by specific monoclonal antibodies.
    • These antigens vary in expression levels across different B cell subpopulations.

    Purpose of the Study:

    • To investigate the role of BLA-1 and BLA-2 antigens in B cell differentiation and lineage commitment.
    • To analyze B cell populations in various mouse models, including xid and nude mice, to understand antigen dynamics.

    Main Methods:

    • Multi-color immunofluorescence analysis using fluorescence-activated cell sorting (FACS).
    • Analysis of B cell subpopulations in normal, CBA/N (xid), and athymic (nude) mice.

    Main Results:

    • Two distinct B cell lineages are identified based on the sequential loss of BLA-1 and BLA-2 during differentiation.

    Related Experiment Videos

  • CBA/N mice exhibit a deficiency in a specific intermediate B cell population (BLA-1+,2-).
  • Nude mice show a reduced proportion of another intermediate population (BLA-1-,2+).
  • Both antigens reappear upon B cell activation, with differential expression in IgM and IgG antibody-producing cells.

    • Conclusions:

    • The differential expression and regulation of BLA-1 and BLA-2 antigens are critical for defining B cell lineages and maturation.
    • These antigens serve as valuable markers for tracking B cell development and activation states.
    • The study provides insights into the immunodeficiency observed in CBA/N and nude mice concerning B cell populations.