A methodology for the characterization of urinary glycosaminoglycans

This study presents a method for the isolation and identification of urinary glycosaminoglycans. Aliquots of 24-hour urine specimens are ultrafiltered through a Biogel filtration column to obtain the macromolecular fractions. These fractions are then subjected to electrophoretic, enzymatic and chemical analyses to obtain qualitative and quantitative information about the glycosaminoglycans contained. The methodology presented is intended to be used in subsequent studies where data can be analyzed and determinations can be made concerning the character of glycosaminoglycans in the urine of stone formers and non-stone formers.