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Early and differential decrease in natural retinoid levels in C57BL/Rij and DBA/2 mice by

A Brouwer, K J van den Berg

    Toxicology and Applied Pharmacology
    |April 1, 1984
    PubMed
    Summary

    3,4,3

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    Area of Science:

    • Environmental Toxicology
    • Biochemistry
    • Pharmacology

    Background:

    • Polychlorinated biphenyls (PCBs) are persistent environmental pollutants with known toxic effects.
    • Retinoids, crucial for various physiological processes, can be affected by xenobiotics.
    • Strain-specific responses to chemical exposure are common in toxicological studies.

    Purpose of the Study:

    • To investigate the dose-dependent effects of 3,4,3',4'-Tetrachlorobiphenyl (TCB) on retinoid levels in different mouse strains.
    • To compare the sensitivity of retinoid reduction as a toxicity marker versus traditional toxicity endpoints.
    • To explore the potential involvement of the mixed function oxidase (MFO) system in TCB-induced retinoid depletion.

    Main Methods:

    • Female C57BL/Rij and DBA/2 mice (5-7 weeks old) were administered weekly intraperitoneal injections of 3,4,3',4'-TCB (1.5-100 mg/kg) for 4 weeks.
    • Retinoid levels (retinol, retinyl palmitate) in liver and serum were quantified.
    • Toxicity parameters including thymus and body weight, liver weight, and aryl hydrocarbon hydroxylase (AHH) activity were assessed.

    Main Results:

    • 3,4,3',4'-TCB induced a dose-dependent decrease in retinoid levels in both mouse strains.
    • C57BL/Rij mice showed significant reductions in liver retinol, retinyl palmitate, and serum retinol.
    • DBA/2 mice exhibited a marked decrease in serum retinol, while other retinoid levels remained largely unaffected.
    • Retinoid level reductions were more pronounced than observed toxicity indicators.
    • Aryl hydrocarbon hydroxylase (AHH) induction did not correlate with retinoid depletion.

    Conclusions:

    • Reduction in retinoid levels is a highly sensitive indicator of polychlorinated biphenyl (PCB) toxicity.
    • The mixed function oxidase (MFO) system is likely not involved in the mechanism of TCB-induced retinoid depletion.
    • Differential strain sensitivity highlights the importance of considering genetic background in toxicological assessments.

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