Some aspects of structure of murine H-2d antigens

The apparent molecular weight of proteins precipitated by normal rabbit serum from lysates of lymph node cells ranged from 10 000 to 150 000. Mono-specific anti-H-2d serum precipitated molecules composed of heavy and light chains with Mr of 47 000 and 12 000, respectively. Tunicamycin treatment caused a decrease of the molecular weight of heavy chain by 4 000 and a change of pI from 5.2 - 5.9 to 5.4 - 5.8. Non-glycosylated heavy chain exhibited Mr of 43 000. The oligosaccharide side chains were resistant to digestion by endo-beta-N-acetylglucosaminidase H. The Mr and pI of beta 2-microglobulin were not altered by treatment with tunicamycin and endo-beta-N-acetylglucosaminidase H. Tunicamycin caused a 50% decrease of overall synthesis of cellular proteins.