Phospholipid and glyceride biosynthesis in 2,4,5,2',4',5'-hexachlorobiphenyl-treated human skin fibroblasts

2,4,5,2',4',5'-Hexachlorobiphenyl (HCB) was taken up by cultured human skin fibroblasts ( A61437 ; GM488 ). HCB caused enhanced incorporation of [2-14C]acetate into phospholipids and glycerides at low concentration and reduced incorporation at high concentrations. sn-[U-14C]Glycerol-3-phosphate incorporation into phospholipids was inhibited. No significant change in total cellular phospholipids was observed. Triglyceride cellular content was increased 29%. The observed stimulation and inhibition of phosphoglyceride synthesis are similar to results obtained with rat liver microsomes.