Related Experiment Videos
Scanning electron microscopy detects bacteria within infection stones
Abstract:
The viability and location of bacteria within infection stones were investigated. Many stones were infected with urea-splitting bacteria, such as Proteus mirabilis and Pseudomonas aeruginosa. Large numbers of bacterial impressions and bodies were found in the interstices surrounded by crystals of apatite and struvite from the nuclei to the peripheral layers. The presence of bacterial colonies even in the nuclear portion of the stones suggests that bacteria participate in the initial stone formation as well as in growth of infection stones. Streptococcus faecalis, a nonurea -splitting bacteria, also was found in some infection stones and probably represents a superimposed infection as a result of changes in bacterial flora owing to treatment with antibiotics.
Insights
Bacteria are present within infection stones, indicating their role in both the initial formation and growth of these stones. This includes urea-splitting bacteria like Proteus mirabilis and Pseudomonas aeruginosa.
Area of Science:
- Microbiology
- Urology
- Crystallography
Background:
- Infection stones are a type of urinary calculus associated with microbial activity.
- Understanding the precise role and location of bacteria within these stones is crucial for effective treatment and prevention.
Purpose of the Study:
- To investigate the viability and spatial distribution of bacteria within infection stones.
- To determine the types of bacteria commonly found in infection stones and their relationship with stone composition.
Main Methods:
- Microscopic examination of infection stones to identify bacterial presence and morphology.
- Analysis of stone composition (apatite and struvite crystals) in relation to bacterial location.
Main Results:
- Urea-splitting bacteria, including Proteus mirabilis and Pseudomonas aeruginosa, were frequently identified in infection stones.
- Bacterial impressions and bodies were observed within the crystal matrix (apatite and struvite) from the stone's nucleus to its periphery.
- Non-urea-splitting bacteria, such as Streptococcus faecalis, were also found, suggesting secondary infections.
Conclusions:
- Bacteria are integral to the formation and growth of infection stones, not merely inhabitants.
- The findings support the hypothesis that bacteria play a role in the initiation of stone formation.
- Antibiotic treatment may alter bacterial flora, potentially leading to superimposed infections by non-urea-splitting bacteria.