Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Differential expression of human interferon genes.

J Hiscott, K Cantell, C Weissmann

    Nucleic Acids Research
    |May 11, 1984
    PubMed
    Summary

    Researchers quantified interferon (IFN) mRNA levels in human cells using S1 mapping. Alpha interferon (IFN-alpha) and beta interferon (IFN-beta) transcript ratios varied by cell type, with distinct IFN-alpha subtypes dominating expression.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Innovative therapy, monoclonal antibodies, and beyond: Highlights from the eighth annual meeting.

    Cytokine & growth factor reviews·2018
    Same author

    Innovative Therapy, Monoclonal Antibodies and Beyond.

    Cytokine & growth factor reviews·2017
    Same author

    The TEL-AML1 fusion protein of acute lymphoblastic leukemia modulates IRF3 activity during early B-cell differentiation.

    Oncogene·2015
    Same author

    The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia.

    Leukemia·2013
    Same author

    Non-beta-lactamase-producing penicillin-resistant Enterococcus faecium in a clinical setting.

    The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses·2012
    Same author

    Enhancing VSV oncolytic activity with an improved cytosine deaminase suicide gene strategy.

    Cancer gene therapy·2011

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Interferons (IFNs) are crucial cytokines involved in antiviral immunity.
    • Understanding the differential expression of IFN subtypes is essential for comprehending immune responses.
    • Accurate quantification of closely related mRNA species presents a technical challenge.

    Purpose of the Study:

    • To develop and apply a method for quantifying multiple, closely related interferon mRNA transcripts.
    • To determine the relative abundance of various alpha interferon (IFN-alpha) subtypes, IFN-beta, and IFN-gamma mRNAs in different human cell types.
    • To investigate how induction methods and cell types influence IFN mRNA expression profiles.

    Main Methods:

    • Development of a quantitative S1 mapping technique for precise mRNA measurement.
    • Analysis of IFN mRNA levels in human peripheral blood leukocytes, lymphoblastoid (Namalwa) cells, HeLa cells, and human fibroblastic cells.
    • Comparison of mRNA expression under various induction conditions.

    Main Results:

    • Significant variation in the ratio of IFN-alpha to IFN-beta transcripts across different cell types.
    • Differential expression patterns of individual IFN-alpha subtypes, with IFN-alpha 1, -alpha 2, and -alpha 4 being the most abundant.
    • Substantial differences in the proportion of specific IFN-alpha mRNA species between cell types, independent of induction protocols.
    • Observed discrepancies between IFN mRNA levels and actual IFN protein production (antiviral activity) in certain cell types.

    Conclusions:

    • The developed S1 mapping method enables accurate quantification of closely related IFN mRNAs.
    • Cell type is a major determinant of IFN-alpha and IFN-beta mRNA expression ratios and specific IFN-alpha subtype abundance.
    • IFN mRNA levels do not always correlate directly with functional IFN production, highlighting post-transcriptional regulatory mechanisms.

    Related Experiment Videos