Related Experiment Videos
Effect of temperature on the survival of Neisseria meningitidis
Abstract:
The effect of different temperatures and variations in temperature on the survival of Neisseria meningitidis in modified Stuart transport medium and in human cerebrospinal fluid (CSF) has been investigated. The meningococcal strains examined died quickly during storage at 37 degrees C, while storage at 4 degrees C and 22 degrees C caused only a moderate decrease in their viability. Short periods of temperature variations during storage at 22 degrees C in transport medium were found to be harmful only when the specimens were kept at -20 degrees C for these short periods. According to our results, 37 degrees C storage temperature cannot be recommended for specimens such as those examined. As 4 degrees C may be harmful to H. influenzae one cannot generally recommend keeping specimens from meningitis patients in refrigerator until analyzed. Room temperature therefore seems preferable. Specimens inoculated onto growth-supporting media should of course contrarily be incubated at 35-37 degrees C.
Insights
Storing Neisseria meningitidis specimens at 37°C significantly reduces survival. Room temperature (22°C) is preferable for transport medium and cerebrospinal fluid (CSF) to maintain viability of meningococcal strains.
Area of Science:
- Microbiology
- Clinical Diagnostics
- Infectious Diseases
Background:
- Neisseria meningitidis is a significant cause of bacterial meningitis.
- Proper sample handling and transport are crucial for accurate diagnosis.
- Temperature stability affects the viability of Neisseria meningitidis in clinical specimens.
Purpose of the Study:
- To investigate the impact of different temperatures and temperature variations on Neisseria meningitidis survival.
- To determine optimal storage conditions for Neisseria meningitidis in transport medium and cerebrospinal fluid (CSF).
Main Methods:
- Survival of Neisseria meningitidis strains was assessed in modified Stuart transport medium and human CSF.
- Specimens were stored at various temperatures: 37°C, 4°C, 22°C, and -20°C.
- Temperature variations were introduced during storage at 22°C.
Main Results:
- Neisseria meningitidis strains exhibited rapid death when stored at 37°C.
- Storage at 4°C and 22°C resulted in a moderate decrease in viability.
- Short temperature fluctuations to -20°C were detrimental, but not at 22°C.
- 37°C is not recommended for storage of these specimens.
Conclusions:
- Room temperature (22°C) appears to be the most suitable condition for storing Neisseria meningitidis specimens in transport medium and CSF.
- Refrigeration (4°C) is not generally recommended due to potential harm to other bacteria like Haemophilus influenzae.
- Incubation at 35-37°C is necessary only for specimens inoculated onto growth-supporting media.