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Related Experiment Videos

Monoclonal antibodies specific for vimentin.

M Osborn, E Debus, K Weber

    European Journal of Cell Biology
    |May 1, 1984
    PubMed
    Summary

    New monoclonal antibodies specifically target vimentin, unlike previous versions. These highly specific vimentin antibodies are valuable tools for cancer diagnostics in pathology.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Cell Biology

    Background:

    • Monoclonal antibodies are crucial tools in biological research and diagnostics.
    • Existing vimentin antibodies often cross-react with other intermediate filament proteins, limiting their specificity.
    • There is a need for highly specific antibodies for accurate cellular and pathological analysis.

    Purpose of the Study:

    • To develop and characterize a set of murine monoclonal antibodies specific for vimentin.
    • To assess the specificity of these antibodies against other intermediate filament proteins.
    • To evaluate the utility of these antibodies in immunofluorescence microscopy and pathological diagnostics.

    Main Methods:

    • Production of monoclonal antibodies using porcine vimentin as antigen.
    • Immunoblot analysis of cell extracts to determine antibody specificity.
    • Immunofluorescence microscopy on tissue sections and cultured cells.
    • Cross-reactivity testing with vimentin from different species (rat, mouse, chicken).

    Main Results:

    • Seven murine monoclonal antibodies were generated that specifically recognize vimentin.
    • These antibodies did not cross-react with desmin or glial fibrillary acidic protein (GFA).
    • Immunofluorescence confirmed vimentin localization in various cell types, including connective tissue, endothelial cells, smooth muscle cells, and astrocytes.
    • Some antibodies exhibited species-specific reactivity.

    Conclusions:

    • The developed monoclonal antibodies are highly specific for vimentin.
    • These antibodies represent a significant advancement, completing a set of specific antibodies for each intermediate filament protein type.
    • Their specificity makes them valuable reagents for pathological applications, aiding in the determination of neoplasm histogenetic origin.

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