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Radioreceptor assay for oxyphenonium.

K Ensing, R A de Zeeuw

European Journal of Respiratory Diseases. Supplement

|January 1, 1984

Summary

A new radioreceptor assay accurately measures oxyphenonium in plasma. This method allows for detailed pharmacokinetic studies of the quaternary anticholinergic drug following inhalation.

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Area of Science:

Pharmacology
Analytical Chemistry
Biochemistry

Background:

Quaternary anticholinergic drugs like oxyphenonium are used therapeutically.
Accurate measurement of drug concentrations in biological fluids is crucial for understanding their effects.
Existing methods may lack the sensitivity or specificity required for certain pharmacokinetic studies.

Purpose of the Study:

To develop and validate a sensitive radioreceptor assay for quantifying oxyphenonium in human plasma.
To enable pharmacokinetic investigations of oxyphenonium, particularly after inhalation administration.

Main Methods:

Development of a radioreceptor assay utilizing the competitive binding of oxyphenonium and 3H-dexetimide to muscarinic receptors.
Implementation of ion pair extraction and reextraction techniques for sample preparation.
Validation of the assay for sensitivity and accuracy in plasma samples.

Main Results:

The developed radioreceptor assay can accurately determine oxyphenonium concentrations in plasma down to 100 pg/ml.
The assay demonstrates sufficient sensitivity for pharmacokinetic studies.
Successful application of the assay to plasma samples after oxyphenonium inhalation.

Conclusions:

A sensitive and specific radioreceptor assay for oxyphenonium in plasma has been successfully developed.
This assay is suitable for pharmacokinetic studies, providing valuable data for drug development and clinical use.
The method facilitates the understanding of oxyphenonium's disposition following inhalation.

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