Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymeric microspheres for immunoresearch.

M Kumakura, I Kaetsu

    Immunological Communications
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    New aldehyde-functionalized microspheres were created using radiation polymerization. These microspheres, with a 1-2 micron diameter, show increased antigen binding with higher glutaraldehyde concentrations, useful for immunoresearch.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    A case of giant cell myocarditis with evidence of cardiac autoimmunity.

    Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
    Same author

    Patents and literature.

    Applied biochemistry and biotechnology·2013
    Same author

    Effect of calcium ions on the irradiation induced inactivation of cellulase.

    Isotopes in environmental and health studies·2011
    Same author

    Spontaneous splitting of a quadruply charged vortex.

    Physical review letters·2008
    Same author

    Comparative morphological study on the tongue and lingual papillae of horses (Perissodactyla) and selected ruminantia (Artiodactyla).

    Italian journal of anatomy and embryology = Archivio italiano di anatomia ed embriologia·2005
    Same author

    Spin squeezing via one-axis twisting with coherent light.

    Physical review letters·2005

    Area of Science:

    • Polymer Chemistry
    • Materials Science
    • Immunotechnology

    Background:

    • Microspheres are crucial in various scientific fields, including diagnostics and drug delivery.
    • Developing microspheres with specific functional groups enhances their utility in targeted applications.
    • Aldehyde groups offer reactive sites for conjugation, vital for immunoassay development.

    Purpose of the Study:

    • To synthesize novel microspheres functionalized with aldehyde groups.
    • To characterize the physical properties, such as particle size and distribution.
    • To evaluate the antigen-binding capacity of the prepared microspheres.

    Main Methods:

    • Radiation polymerization of acrolein in the presence of hydroxyethyl methacrylate and glutaraldehyde.
    • Particle size analysis to determine the average diameter and distribution.

    Related Experiment Videos

  • Assessing antigen-binding ability by varying glutaraldehyde concentration.

    • Main Results:

    • Successfully prepared microspheres with functional aldehyde groups.
    • Achieved narrow size distribution with an average particle diameter of 1-2 microns.
    • Demonstrated a positive correlation between glutaraldehyde concentration and antigen-binding ability.

    Conclusions:

    • A simple and effective method for preparing aldehyde-functionalized microspheres was established.
    • The microspheres exhibit desirable particle characteristics for potential applications.
    • These microspheres show promise for use in various immunoresearch applications.