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Conjugal plasmids in group D streptococci.

M Bondi, P Messi, V Borghi

    Microbiologica
    |April 1, 1984
    PubMed
    Summary
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    Group D streptococci often carry plasmids conferring antibiotic resistance, which can be transferred between bacteria. These plasmids also influence hemolytic and bacteriocin activities, impacting bacterial interactions.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Group D streptococci are significant human pathogens.
    • Plasmids are extrachromosomal DNA elements that can carry virulence factors and antibiotic resistance genes.
    • Understanding plasmid-mediated traits in streptococci is crucial for clinical management.

    Purpose of the Study:

    • To investigate the plasmid content of clinical isolates of Group D streptococci.
    • To characterize plasmid-associated drug resistance, hemolytic activity, and bacteriocin production.
    • To explore the transferability of these traits via conjugation.

    Main Methods:

    • Isolation and characterization of 159 Group D streptococci strains from clinical specimens.
    • Plasmid DNA extraction and analysis.

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  • Antibiotic susceptibility testing.
  • Bacterial conjugation experiments to assess trait transferability.
  • Hemolytic activity assays.
  • Bacteriocin typing.

    • Main Results:

    • 73.6% of strains exhibited antibiotic resistance.
    • Drug resistance was transferable by conjugation in 69% of resistant strains.
    • Three distinct types of transconjugants with varying hemolytic activity were identified in S. faecalis subsp. zymogenes.
    • Bacteriocin activity was detected and classified into different types.

    Conclusions:

    • Plasmids in Group D streptococci frequently carry transferable antibiotic resistance genes.
    • Plasmid-borne genes influence hemolytic activity and bacteriocin production.
    • These findings highlight the role of plasmids in streptococcal virulence and adaptability.