Changes of apolipoprotein B molecular weight and immunoreactivity in malondialdehyde-modified low density...

ApoB molecular weight by SDS polyacrylamide gel electrophoresis and its immunoreactivity by either electroimmunoassay or radial immunodiffusion has been recorded in human malondialdehyde-modified low density lipoproteins. Malondialdehyde treatment of LDL leads to a reduction of the typical B-100 band and to the appearance of two heavier peptides that can be designated as B-126 and B-151 according to the centile nomenclature. A significant increase of the height of apoB rockets by electroimmunoassay and of the immunoprecipitates diameter by radial immunodiffusion was also recorded.