Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human lung tryptase. Purification and characterization.

T J Smith, M W Hougland, D A Johnson

    The Journal of Biological Chemistry
    |September 10, 1984
    PubMed
    Summary

    Researchers purified and characterized human lung tryptase, a mast cell enzyme. This study details its properties, inhibitors, and localization within mast cell granules.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Donepezil treatment restores the ability of estradiol to enhance cognitive performance in aged rats: evidence for the cholinergic basis of the critical period hypothesis.

    Hormones and behavior·2009
    Same author

    Virtual enriched environments in paediatric neuropsychological rehabilitation following traumatic brain injury: Feasibility, benefits and challenges.

    Developmental neurorehabilitation·2009
    Same author

    Salivary secretion, mucin concentrations and Candida carriage in HIV-infected patients.

    Oral diseases·2009
    Same author

    High genetic diversity, phenotypic uniformity, and evidence of outcrossing in sclerotinia sclerotiorum in the columbia basin of washington state.

    Phytopathology·2008
    Same author

    Competitive Fitness of Phytophthora infestans Isolates Under Semiarid Field Conditions.

    Phytopathology·2008
    Same author

    Aggressiveness of Isolates of Phytophthora infestans from the Columbia Basin of Washington and Oregon.

    Phytopathology·2008

    Area of Science:

    • Biochemistry
    • Immunology
    • Cell Biology

    Background:

    • Human lung tryptase is a serine protease released by mast cells.
    • Understanding its enzymatic properties is crucial for studying allergic and inflammatory responses.

    Purpose of the Study:

    • To isolate and extensively characterize human lung tryptase.
    • To investigate its enzymatic activity, inhibitor profile, and cellular localization.

    Main Methods:

    • Purification from human lung tissue using heparin gradient elution from cellulose phosphate.
    • Enzyme activity assays, gel filtration, SDS-PAGE, amino acid analysis.
    • Generation of antisera for immunohistochemical staining.

    Main Results:

    • Isolated active tryptase species with Mr 30,900 and 31,600.
    • Enzyme activity is sensitive to NaCl concentration and unaffected by natural serum inhibitors.
    • High tryptophan content and localization to mast cell granules confirmed.

    Conclusions:

    • Human lung tryptase is a distinct trypsin-like protease.
    • Its properties suggest a role in inflammatory processes.
    • Immunohistochemistry confirms mast cell granule localization.

    Related Experiment Videos