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The catalytic element of a ribosomal RNA-processing complex.

B Pace, D A Stahl, N R Pace

    The Journal of Biological Chemistry
    |September 25, 1984
    PubMed
    Summary

    The Bacillus subtilis RNase M5 complex

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    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • The Bacillus subtilis RNase M5 complex is crucial for 5S rRNA maturation.
    • It comprises ribosomal protein BL16 and a catalytic alpha component.

    Purpose of the Study:

    • To identify the catalytic element within the RNase M5 complex.
    • To elucidate the role of ribosomal protein BL16 in the RNase M5 reaction.

    Main Methods:

    • Purification of the RNase M5 alpha component.
    • Polyacrylamide gel electrophoresis to identify the active polypeptide.
    • Enzyme activity assays under varying reaction conditions, including dimethyl sulfoxide.

    Main Results:

    • The catalytic activity of RNase M5 was demonstrated to be independent of ribosomal protein BL16 under specific conditions (20-30% DMSO).
    • The alpha component was confirmed as the actual catalytic nuclease (RNase M5).
    • Kinetic analyses indicated minimal energetic contribution from the alpha-BL16 association.

    Conclusions:

    • The alpha component is the sole catalytic entity of RNase M5.
    • Ribosomal protein BL16 likely functions as a scaffold, facilitating substrate recognition by the nuclease.

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